NEBNext Single Cell/Low Input cDNA Synt from New England Biolabs

Product Specs
ItemNEBNext Single Cell/Low Input cDNA Synt
CompanyNew England Biolabs
Price Pricing Info Supplier Page View Company Product Page
Catalog NumberE6421S
Quantity24 reactions
ApplicationsDNA Analysis, NGS
Brand/TrademarkNew England Biolabs Inc (NEB)
Temperature-20 C

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New England Biolabs

240 County Road

Ipswich, Massachusetts 1938

United States

Phone: 800-632-5227
800-632-7799
978-921-1350

Company Profile

Website: http://www.neb.com

(1) LINE-1 retrotransposons drive human neuronal transcriptome complexity and functional diversificationScience AdvancesNovember 3, 2023Raquel Garza, Diahann A. M. Atacho, Anita Adami, Patricia Gerdes, Meghna Vinod, PingHsun Hsieh, Ofelia Karlsson, Vivien Horvath, Pia A. Johansson, Ninoslav Pandiloski, et al.October 2019) using the NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module (catalog nos. E6421S for 24 reactions and E6421L for 96 reactions, New England Biolabs), the Iso-Seq Express Oligo Kit (catalog

(2) Long read sequencing to reveal the full complexity of a plant transcriptome by targeting both standard and long workflowsPlant MethodsOctober 21, 2023Othman Al-Dossary, Agnelo Furtado, Ardashir KharabianMasouleh, Bader Alsubaie, Ibrahim Al-Mssallem, Robert J. Henryof the Iso-Seq library, the NEBNext Single Cell/Low Input cDNA Synthesis, Amplification Module (NEB, E6421S), and the SMRTbell Express Template Prep Kit 2.0 (PacBio, 100-938-900) were used based on the standard

(3) Einkorn genomics sheds light on history of the oldest domesticated wheatNatureAugust 2, 2023Hanin Ibrahim Ahmed, Matthias Heuberger, Adam Schoen, Dal-Hoe Koo, Jesus Quiroz-Chavez, Laxman Adhikari, John Raupp, Stéphane Cauet, Nathalie Rodde, Charlotte Cravero, et al.separately using NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module (New England Biolabs, E6421S). The ProNex Size-Selective Purification System (Promega, NG2001) was used for size selection. One SMRT

(4) Direct haplotype-resolved 5-base HiFi sequencing for genome-wide profiling of hypermethylation outliers in a rare disease cohortNature CommunicationsMay 29, 2023Warren A. Cheung, Adam F. Johnson, William J. Rowell, Emily Farrow, Richard Hall, Ana S. A. Cohen, John C. Means, Tricia N. Zion, Daniel M. Portik, Christopher T. Saunders, et al.cDNA synthesis with a NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module (NEB, Cat. No. E6421S) and an Iso-Seq Express Oligo Kit (PacBio, Cat. No. 101-737-500) as described in the Iso-Seq Express

(5) The gyrfalcon (Falcon rusticolus) genomeG3 Genes|Genomes|GeneticsMarch 9, 2023Andrea Zuccolo, Sara Mfarrej, Mirko Celii, Saule Mussurova, Luis F Rivera, Victor Llaca, Nahed Mohammed, Arnab Pain, Abdulmajeed Fahad Alrefaei, Abdulwahed Fahad Alrefaei, et al.was done using NEBNext Single Cell/Low input cDNA synthesis and amplification module (NEB, Cat No.: E6421S) with Iso-Seq Express Oligo Kit (Pacbio, 101-737-500) and Iso-seq library was built with SMRTbell Express

(6) A chromosome-level reference genome and pangenome for barn swallow population genomicsCell ReportsJanuary 31, 2023Simona Secomandi, Guido R. Gallo, Marcella Sozzoni, Alessio Iannucci, Elena Galati, Linelle Abueg, Jennifer Balacco, Manuela Caprioli, William Chow, Claudio Ciofi, et al.NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module (New England BioLabs, cat. no. E6421S) and Iso-Seq Express Oligo Kit (PacBio PN 10 1-737-500) from 300 ng of total RNA for both brain and

(7) NAb-seq: an accurate, rapid, and cost-effective method for antibody long-read sequencing in hybridoma cell lines and single B cellsmAbsDecember 31, 2022Hema Preethi Subas Satish, Kathleen Zeglinski, Rachel T. Uren, Stephen L. Nutt, Matthew E. Ritchie, Quentin Gouil, Ruth M. Kluckcells were prepared using the NEBNext® single cell/low input cDNA synthesis and amplification module (E6421S; New England BioLabs Inc.). As a single mammalian cell contains only 0.1 pg mRNA,42 this module is required

(8) Single-cell analysis and functional characterization uncover the stem cell hierarchies and developmental origins of rhabdomyosarcomaNature CancerAugust 18, 2022Yun Wei, Qian Qin, Chuan Yan, Madeline N. Hayes, Sara P. Garcia, Haibin Xi, Daniel Do, Alexander H. Jin, Tiffany C. Eng, Karin M. McCarthy, et al.the NEBNext Single Cell/Low Input cDNA Synthesis and Amplification Module kit (New England Biolabs # E6421S). Amplified cDNA was then used in each quantitative PCR (PowerUp SYBR Green Master Mix, Fisher Scientific

(9) Long-read genome sequencing of bread wheat facilitates disease resistance gene cloningNature GeneticsMarch 14, 2022Naveenkumar Athiyannan, Michael Abrouk, Willem H. P. Boshoff, Stéphane Cauet, Nathalie Rodde, David Kudrna, Nahed Mohammed, Jan Bettgenhaeuser, Kirsty S. Botha, Shannon S. Derman, et al.using the NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module (New England Biolabs, E6421S) and the ProNex Size-Selective Purification System (Promega, NG2001) for size selection. In brief, 300

(10) Single worm transcriptomics identifies a developmental core network of oscillating genes with deep conservation across nematodesGenome ResearchSeptember 1, 2021Shuai Sun, Christian Rödelsperger, Ralf J. Sommerliquid nitrogen. Afterward, 3 µL of lysis buffer (low input cDNA synthesis & amplification module; NEB E6421S) was added, and samples were incubated for 40 min at 65°C, followed by 1 min at 85°C. To remove genomic

(11) Identification of neural progenitor cells and their progeny reveals long distance migration in the developing octopus braineLifeAugust 24, 2021Deryckere A, Styfhals R, Elagoz AM, Maes GE, Seuntjens E.kit,NEBNext Single Cell/Low Input cDNA Synthesis and Amplification Module,New England BioLabs.,Cat#: E6421S, Commercial assay or kit,Superscript III Reverse Transcriptase,Invitrogen,Cat#: 18080–044, Commercial

(12) Reference genome and demographic history of the most endangered marine mammal, the vaquitaMolecular Ecology ResourcesMay 1, 2021Phillip A. Morin et al.reverse transcribed using the NEBNext Single Cell/Low Input cDNA Synthesis & Amplification Module (NEB E6421S) from 238 ng total RNA. Amplified cDNA was cleaned with 86 µl ProNex beads. The PacBio Iso‐seq library

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